Sometimes the experimenter is interested in examining each postsynaptic response evoked by stimulus pulses in a train, in which case the baseline and synaptic response of each pulse is analyzed in the normal manner shown in this figure.  Alternatively, the synaptic responses evoked by train stimulation can be treated as a whole train object in a special manner by WinLTP.

Analyze every pulse in train using the baseline of first pulse as the baseline for each pulse

First, the synaptic responses to each train pulse may be analyzed relative to the baseline of the first pulse in the train as shown in the figure below.

Analysis of every EPSP using the baseline of the first pulse.  This figure shows the peak amplitude measurement of each fEPSP in a 4 pulse train relative to the prestimulus baseline of the first pulse.  Note the four measurements in the spreadsheet, one for each pulse.

Analyze the whole train by analyzing only first pulse in train but detecting the whole train

Alternatively, if the baseline and response of only the first train pulse is used, all stimulus artifacts are blocked, and the time of measurement is set sufficiently long to encompass the whole train, then the synaptic response of the entire train will be measured.  With this analysis, the peak amplitude of the largest EPSP in the train and the area of the  synaptic response of the entire train can be obtained.  Blanking of stimulus artifacts is necessary to permit accurate calculation of area and peak amplitude without contamination by stimulus artifacts occurring near the fEPSP peak.  Note the one measurement in the spreadsheet, one for the first pulse, e.g. the whole train.

Analysis of trains as a single entity by using the baseline and response of only the first pulse in the train, but with the time of measurement set to a sufficiently long duration after the first stimulus pulse (3 to 55 ms here) to encompass the whole train  This measures the peak amplitude of the largest fEPSP in the train and the area of the entire synaptic response of the train.