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Fast Repeat (LTD) Sweep
Stimulation with No Time Between Sweeps |
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Sometimes is is useful, particularly when
recording long duration EPSCs during Fast Repeat (LTD) 1 or 2 Hz
sweep (and pulse) stimulation, to record the whole 1000 or 500
msec sweep so as to record all of the EPSC tail current.
In some data acquisition systems, it is
impossible to stimulate and record whole contiguous sweeps (with no
time delay between them) because of the time required to save the
sweep before starting to acquire and stimulate another sweep.
In WinLTP's multitasking/multithreaded program,
acquisition and stimulation
output of the current sweep can procede at the same time as saving
of the preceeding sweep.
The figure below shows an example of this
contiguous sweep stimulation and acquisition using Fast Repeat Sweep
stimulation produced by 4 contiguous 1000 msec long P0sweeps every 1
second. This was produced by the following Protocol Builder
script:
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The traces in the bottom P0 Sweep
Stimulation graph show a 1000 msec duration P0sweep with 1
S0 pulse/sweep and 20 S1 pulses/sweep at 50 msec pulse
interval. The traces in the middle P0 Stimulus Sweep
Acquisition graphs shows the recording of the last
1000 msec duration P0sweep showing the one S0 pulse in the
AD0 trace and the 20 S1 pulses in the AD1 trace. The
top traces in the Continuous Acquisition graphs show the
output recorded for the S0 pulse at 1/sec in the AD0 trace
and the S1 pulses at 20/sec in the AD1 trace. |