WinLTP
Version 2.20b
Leading-Edge Features
Special Features
Ordinary Features
Additional Features
Overview of WinLTP

This figure shows the basic layout of the WinLTP program showing Protocol fields (upper left panel), Analysis graphs (one slope graph) (top right panel), Sweep Acquisition (middle right panel), Sweep Stimulation fields and graphs (lower left and right panels), and the Spreadsheet and Run Buttons (bottom panels).

 

Basic layout of the WinLTP Acquisition program.  Detection fields to change synaptic potential detection values, as well as, the Imposing, Linking and Log tabsheets are hidden behind the Protocol tabsheet.

 

This figure shows a basic LTP experiment of slow alternating dual pathway stimulation, in this case using signal averaging. The right middle graph shows an extracellular synaptic response from the CA1 region of the hippocampus, averaged from four sweeps, and produced by single extracellular S0 stimulus pulses, 10 ms from the start of the sweep. Superimposed on the synaptic waveform are red lines to show where calculations were made for the S0 slope. The slope  'calculation lines' are color-coded and are shown in red for an S0-evoked fEPSP and magenta for an S1-evoked fEPSP. The right top graph shows calculations for slope produced by S0 stimulation (red triangles) and S1 stimulation (magenta squares).

In WinLTP, alternating dual pathway stimulation (S0 then S1) of the experiment is achieved by producing dual alternating sweeps (Pulse P0sweep then Pulse P1sweep) in which the P0sweep has one pathway stimulation by one extracellular electrode, S0, and the P1sweep has one pathway stimulation by extracellular electrode S1. The MainProtocol panel shows the alternating P0sweep every 30 sec, then P1sweep every 30 sec, in an AvgLoop of 4 to produce an average every 4 sweeps.  

LTP induction by S0 stimulation (indicated by 'LTP' and up arrow below red triangles in the right top panel) is produced by  by clicking the ‘Single T0’ Run Button to evoke a single Train Sweep of 100 S0 pulses at 100 Hz.

 The induction of LTD by S0 stimulation (indicated by 'LTD' below red triangles in the top panel) is initiated by clicking the ’Repeat P0’ Run Button which produces rapidly repeating Pulse P0sweeps for a set number of times (900 here), once a second here. LTD induction by S1 stimulation is simlarly later produced.

 

Of course, WinLTP functions are not limited to extracellular synaptic local field potential recording.  It is also very good for patch-clamping, including being able to accurately sure series resistance from the unfiltered trace while measuring peak amplitude from the filtered trace

The ability to multitask and perform stimulus/acquisition sweeps while at the same time record gap-free, continuous acquisition Axon Binary Files means that you can run mini acquisiton experiments by recording gap-free, continuous acquisition data (for later mini anaysis), while simultaneously assessing patch electrode viability and cell heath by measuring patch electrode series resistance and cell input resistance.

And with the Protocol Builder you can generate complex stimulation protocols like those for in-vitro kindling in epilepsy studies, or, when coupled with automated perfusion, enable running nearly fully automated experiments.

And the imposing of single and averaged sweeps enables careful visual examination of synaptic waveforms not only after, but also during, the experiment.