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| Running WinLTP Acquisition in the
Advanced Mode (to use the Protocol Builder, , Experimental Log
file, converting ADsweep files to Axon Binary Files, and full multitasking)
requires the purchase
of a License Key. The cost for the License Key
is $1250 or �800 for up to 5 copies.
We hope that you will support
WinLTP by
purchasing an Advanced Mode License. This helps us to provide
technical support of Basic Mode users throughout the world, and to
continue further WinLTP development.
Running WinLTP Acquisition
in the Basic Mode continues to
be free. (Currently,
there are no plans to charge for future versions of WinLTP for running in the
Basic Mode. However future changes
to this policy cannot be ruled out.)
Commercial users are required to buy an Advanced Mode license to use
WinLTP in either the Basic and/or Advanced Modes.
Data reanalysis with WinLTP Reanalysis
program continues to be free.
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WinLTP is a stimulation, data acquisition and on-line
analysis electrophysiology software program for studying Long-Term Potentiation (LTP), Long-term
Depression (LTD), and related phenomena. WinLTP is multitasking and
simultaneously runs 1) LTP stimulus/acquisition/analyzing sweeps,
and 2) continuous acquisition saving Axon Binary Files (abf).
WinLTP runs
on Windows PCI and PCIexpress slot computers
and uses National Instruments PCI and PCIexpress M- and X-Series boards and Axon Instruments' Digidata
1320A and 1322A data acquisition boards.
Other software that can use the M-Series boards includes Axograph
Scientific's AxoGraph X, WaveMetrics' IGOR,
National Instruments' LabView, John Dempster's Strathclyde Electrophysiology
Suite (WinWCP
and WinEDR), Silver lab's Nclamp,
QUB data acquisition, and data
acquisition programs such as mPhys
written in MatLab.
The basic design philosophy behind WinLTP is to provide
simple stimulation protocols using the LTP Protocols in the Basic Mode, or more complex
stimulation protocols using Protocol Builder in the Advanced mode, do sufficient online analysis to
let you modify the experiment as it runs (such as changing baselines or protocol
flow), do simultaneous continuous acquisition, and use inexpensive but excellent
data acquisition boards (National Instruments M- and X-Series boards).
WinLTP
on/off-line analyses include basic analyses of synaptic potentials (Peak Amplitude, Latency, Slope, Area, Duration, Rise/Decay Time,
Coastline, PopSpike
Amplitude and Latency, Average Amplitude), and also Cell Resistance (Rm), and
Patch Electrode Series Resistance (Rs).
However, WinLTP is not designed to
do every possible synaptic analysis, and instead is designed to work with other
very good, and often inexpensive analysis programs. For example,
additional synaptic event analyses such as synaptic exponential decay time can
be done by separate analysis programs including Synaptosoft's MiniAnalysis
and Christoph Schmidt-Hieber's StimFit32,
and spontaneous synaptic events in continuous acquisition gap-free abf
files can be analyzed by William Heitler's DataView,
Synaptosoft's MiniAnalysis, Axograph
Scientific's AxoGraph X and Molecular
Device's ClampFit.
If you have any questions please contact WinLTP
support at: [email protected].
| More Complex WinLTP functionality |
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1. |
Use
the Protocol Builder
to build complicated protocols by pulling
down �building blocks� such as Loops, Delays, Runs and Sweeps from
User Interface buttons. The LTP Protocols can implement the simple
protocols used in LTP experiments. |
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2. |
Multitasking |
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a. Repeat sweep stimulation, acquisition and
analysis
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b. Tape recorder (Continuous Acquisition of 2 AD channels down
to 25 usec sample intervals, saved to a gap-free Axon Binary File)
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3. |
Fast Repeat (LTD) Sweep Stimulation with no time between sweeps
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4. |
On and off-line calculation and plotting of several
waveform parameters: DC baseline, Peak Amplitude, Latency, Slope, Maximum
Slope, Area, Duration, Rise Time, Decay Time, Coastline, PopSpike
Amplitude and Latency, Average Amplitude, Cell
resistance (Rm), and Patch electrode series resistance (Rs) |
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5. |
Analyze all S0- and S1-evoked
synaptic responses in
a
sweep
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6. |
Special analyses of trains including: Analyze all peaks in a train
relative to the baseline of the first pulse
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7. |
Automatic blanking of stimulus artifacts
to allow accurate determination of peaks and areas in a train
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8. |
An Experimental
Log to show when Loops, Runs and Sweeps have occurred, when
solutions were changed (inputting the information by keyboard), and also
prints Detection,
Stimulation or All Protocol Values to the Log
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9. |
Measurement of Patch Electrode Series Resistance (Rs)
using Rs peak, or Rs
single or double exponential curve fitting |
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10. |
Online
measurement of Rs from the
unfiltered trace, and the synaptic Peak Amplitude from the filtered
trace (reduces necessity of reanalysis). |
| Simple WinLTP functionality |
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1. |
The WinLTP records synaptic activity in
extracellular, intracellular or patch clamp modes |
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2. |
2 AD channel acquisition (down to 25 usec sample interval)
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3. |
Two extracellular stimulation outputs (S0 and S1) |
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4. |
Two simultaneous patch-clamp recordings using two analog
outputs |
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5. |
Analog
stimulation including analog
trains and ramps (loop within loop stimulation) |
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6. |
Repetitive sweeps with simultaneous data acquisition (up
to 1,000,000 samples and 100 sec duration) and stimulation (using two
extracellular pathway stimulation, S0 and/or S1, and epoch-like digital
and intracellular analog stimulation) |
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7. |
Basic LTP Protocols are either slow single pathway S0
stimulation, or slow alternating dual pathway (S0 then S1) stimulation |
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8. |
Sweeps can be signal averaged,
stimulus artifact blanked, and/or digitally
filtered on-line and off-line |
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9. |
LTP induction can be produced by: Single train, repetitive train (theta burst stimulation), and
primed
burst stimulation |
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10 |
LTD stimulation and analysis can be performed using fast
repetitive single pulse sweeps (at up to 10 Hz), or several pulses in a
sweep for faster repetitive stimulation |
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11. |
Patch
sealtest protocol implemented
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12. |
Save ADsweep Graph as a Windows Enhanced Metafile
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13. |
Save your Spreadsheet/ AmpFile data to an Excel XLS file
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14. |
Reanalyze straight ASCII files (skip header)
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15. |
Automatic data folder creation at startup
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16. |
WinLTP Reanalysis works on Macs with Intel
processors
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17. |
On-line acquisition runs in Windows XP,
Vista and 7 (M- and X-Series boards), and in Windows 2000 and XP (Digidata 132x boards)
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| Additional WinLTP functionality |
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1. |
Convert WinLTP ADsweep files
to Axon
Binary (*.abf) Files |
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2. |
Multiple WinLTP programs and
AD boards can now be run on one computer. |
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1. |
For Digidata 132x boards, a PCI
slot computer or laptop computer (3 GHz or
higher processor
recommended) |
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For M- or X-Series boards, PCI
or PCIexpress slot computer with at least a
2.8 GHz Pentium 4 processor (e.g. with hyperthreading, the faster the
better, multi-core processors supported) (M-Series USB 2.0 boards not supported) |
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2. |
For M- and X-Series boards, Windows XP or Vista; for Digidata 132x boards, Windows 2000 or XP |
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3. |
2048 MB of memory recomended (512 MB minimum) |
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4. |
1280x1024 pixel monitor recommended (1024x768 minimum) |
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5. |
Data acquisition boards |
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a. |
Molecular Devices' Digidata 1320A or 1322A
(for WinLTP, NI boards work much better than Digidata boards) |
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b. |
National Instruments M-Series
PCI boards (PCI-6221, PCI-6229, PCI-6251 or PCI-6259), M-Series
PCIexpress boards (PCIe-6251 or PCIe-6259), and X-Series
PCIexpress boards (PCIe-6321, PCIe-6323, PCIe-6351,
PCIe-6353, PCIe-6361 or PCIe-6363) |
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c. |
USB
2.0 M-Series boards can also be used with Dual- and QuadCore computers |
The free DOS LTP Program
is still available (although the free Basic Mode of WinLTP does everything that
the LTP Program does, and much, much more).
Copyright � WinLTP Ltd. and The University of
Bristol, 1991-2010. All Rights Reserved.
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| Updated December 17, 2010. |
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